No, I got the trophy on my good playthru. You need ALL neutral powers, and all Karma powers for the path you choose. (If you started good,you just need the good karma powers. and vice-versa for evil).
Also if you need help with other Trophies, I HIGHLY recommend using this Trophy Guide.
From PS3Trophies.org, By StarGate55555. It's nicely laid out and very detailed.
If you don't already have an account you might go ahead and sign up, The community is great, and ALL games with Trophies have detailed guides.
Primalcarnage is right. You might like to know, however, that once you get both endings, you are given access to all powers, regardless of karma. So fun. You don't get the powers that are specifically tied to your karma level, but that's all, and it'd be a LOT of work to change your karma from full evil/good to the other end of the spectrum.
Either way everyone can get the trophy just playing the game twice through. =]