U.N. Owen Was Her, by ZUN. From perhaps one of my favorite game series.
The melody kicks in around the 30 second mark.
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Kind of a recent toss up between these few (Yes, I listen to TONS of music)
1) Type O Negative - September Sun, Everything Dies, I Like Goils (love it), Love You To Death, & Black No. 1
2) Burn Halo - Threw It All Away, Dakota, Stuck In A Rut, & We Won't Live Forever
3) Eighteen Visions- Vanity, I Let Go, Motionless and White, & Flowers for Ingrid
4) Killswitch Engage - Unbroken, & Life to Lifeless
5) Marilyn Manson - In The Shadow of the Valley of Death, Coma White, The Speed of Pain, Running to the Edge of the World
6) Godsmack- Moon Baby, Stress, Bad Religion, I Stand Alone, Realign, Spiral, & I F****** Hate You
7) James Blunt (no laughing lol) - Tears & Rain, You're Beautiful, & Goodbye My Lover
8) Rise Against - My Life Inside Your Heart, Roadside, The Good Left Undone, & Make This Stop
9) Machine Head- Locust & Halo
10) The Offspring - Gone Away, Come Out and Play
11) Deftones - Change (In the House of Flies
12) Avenged Sevenfold- I Won't See You Tonight (both parts), To End The Rapture, Radiant Eclipse, & Shattered by Broken Dreams